Download e-book for kindle: A Platonic Pythagoras. Platonism and Pythagoreanism in the by Mauro Bonazzi, Carlos Levy, Carlos Steel

By Mauro Bonazzi, Carlos Levy, Carlos Steel

ISBN-10: 2503519156

ISBN-13: 9782503519159

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Green, E. Sugg, R. Anderegg, D. S. Millington, D. L. Norwood, J. McGeehan, and J. Wiseman, J. Bioi. Chern. 267, 1434 (1992). [2] FLUORIMETRIC ASSAYS OF PROTEOLYTIC ENZYMES 33 but substrates with two cleavages can be useful in the earlier stages of an investigation. In such a case, it may be possible to suppress one cleavage by introducing anN-methyl or a /3-branched amino acid at the scissile bond. Notes Added in Proof Synthetic Peptide Libraries Perhaps the most exciting new development has been the application of synthetic peptide libraries59·60 to the study of peptidase specificity.

Acta 1004, 20 (1989). 4 A. J. Birkett, D. F. Soler, R. L. Wolz. J. S. Bond, J. Wiseman, J. Berman, and R. B. Harris, Anal. Biochem. 196, 137 (1991). 5 J. R. Petithory, F. R. Masiarz, J. F. Kirsch, D. V. Santi, and B. A. Malcolm, Proc. Nat!. Acad. Sci. A. 88, 11510 (1991). 6 V. Schellenberger, R. A. Siegel, and W. J. Rutter, Biochemistry 32, 4344 (1993). METHODS IN ENZYMOLOGY, VOL. 248 Copyright © 1995 by Academic Press, Inc. All rights of reproduction in any form reserved. 36 [3] METHODS Sample HPLC System ~ Mass Spectrometry ion Source 1 CF-Probe - - Fused Silica FIG.

L. Stein, Biochemistry 31, 12618 (1992). 50 R. P. Haugland, "Handbook of Fluorescent Probes and Research Chemicals," p. 24. Molecular Probes, Eugene, Oregon, 1985. 30 METHODS [2] though the Mea and Dnp groups are separated by 9 amino acid residues, the cleavage is accompanied by a 20-fold increase in fluorescence. The coumarin-derived peptides described above were labeled with the fluorescent group by acylation of the amino terminus. In some circumstances it may be necessary to leave the terminus as a free amine or to use it for the attachment of a solubilizing group.

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A Platonic Pythagoras. Platonism and Pythagoreanism in the Imperial Age by Mauro Bonazzi, Carlos Levy, Carlos Steel

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